Hotspot

trnT-GGU-psbD

LSC · 33837-35409 · 1573 bp

Back to Vauquelinia

Pi 0.0241

MarkerSeek score 77.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1573
variable_sites: 50
pi: 0.0241
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1629-1787 bp	1.0	85.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1632-1790 bp	1.0	85.6
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1631-1789 bp	1.0	85.6
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1619-1777 bp	1.0	85.5
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1634-1792 bp	1.0	85.4