Hotspot

psbM-trnD-GUC

LSC · 31279-32476 · 1198 bp

Back to Vauquelinia

Pi 0.00501

MarkerSeek score 38.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 10
pi: 0.00501
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGCATTCCGACGAAGAAGTC	CCCCAATCCCAATACGGAAG	50.0	55.0	58.0	58.0	188-2258 bp	0.5	61.7
psbM-trnD-GUC_p2	AGCATTCCGACGAAGAAGTC	CAATAATTTGCCGATCCGCC	50.0	50.0	58.0	57.9	188-2322 bp	0.5	61.4
psbM-trnD-GUC_p3	AGCATTCCGACGAAGAAGTC	CCAATAATTTGCCGATCCGC	50.0	50.0	58.0	57.9	188-2323 bp	0.5	61.4
psbM-trnD-GUC_p4	AGCATTCCGACGAAGAAGTC	ACCCATTTGTTGCTCTCCAG	50.0	50.0	58.0	58.1	188-2290 bp	0.5	61.2
psbM-trnD-GUC_p5	AGCATTCCGACGAAGAAGTC	ATAATTTGCCGATCCGCCAT	50.0	45.0	58.0	58.1	80-2320 bp	0.5	61.2