Hotspot

ccsA

SSC · 121693-122655 · 963 bp

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Pi 0.00415
MarkerSeek score 35.80
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
963
variable_sites
8
pi
0.00415
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TATCAGGTGAGAGGGTCGAC TGGAATTGGTAGACACGCTG 55.0 50.0 57.9 57.9 1420-1428 bp 1.0 80.7
ccsA_p2 TATCAGGTGAGAGGGTCGAC GTGGAATTGGTAGACACGCT 55.0 50.0 57.9 57.9 1421-1429 bp 1.0 80.7
ccsA_p3 ACTATCAGGTGAGAGGGTCG TGGAATTGGTAGACACGCTG 55.0 50.0 57.9 57.9 1422-1430 bp 1.0 80.7
ccsA_p4 ACTATCAGGTGAGAGGGTCG GTGGAATTGGTAGACACGCT 55.0 50.0 57.9 57.9 1423-1431 bp 1.0 80.7
ccsA_p5 TATCAGGTGAGAGGGTCGAC TAGACACGCTGCTCTTAGGA 55.0 50.0 57.9 57.9 1411-1419 bp 1.0 80.5