Hotspot

trnL-UAG-ndhF

SSC · 122822-125471 · 2650 bp

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Pi 0.0114
MarkerSeek score 58.10
Primer available yes
Label trnL-UAG-ndhF

Feature Metadata

label_name
trnL-UAG-ndhF
feature_type
igs
region
SSC
length_bp
2650
variable_sites
61
pi
0.0114
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ndhF_p1 CAGCGTGTCTACCAATTCCA TTGGAAGTGGAATGAAGGGT 50.0 45.0 57.9 56.6 2597-2732 bp 1.0 74.1
trnL-UAG-ndhF_p2 AGCGTGTCTACCAATTCCAC TTGGAAGTGGAATGAAGGGT 50.0 45.0 57.9 56.6 2596-2731 bp 1.0 74.1
trnL-UAG-ndhF_p3 TCCTAAGAGCAGCGTGTCTA TTGGAAGTGGAATGAAGGGT 50.0 45.0 57.9 56.6 2606-2741 bp 1.0 73.9
trnL-UAG-ndhF_p4 AGAAACACAATGCCGCTACT TTGGAAGTGGAATGAAGGGT 45.0 45.0 57.8 56.6 2657-2792 bp 1.0 73.4
trnL-UAG-ndhF_p5 CTAAGAGCAGCGTGTCTACC TTGGAAGTGGAATGAAGGGT 55.0 45.0 57.8 56.6 2604-2739 bp 1.0 73.3