Hotspot

rps12-clpP

LSC · 70523-70898 · 376 bp

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Pi 0.0967
MarkerSeek score 72.50
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
376
variable_sites
59
pi
0.0967
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.81
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 TGCACCAACGGAAACCATAA ATGTCAGCCACAGAAGCAAA 45.0 45.0 58.0 58.0 597-735 bp 1.0 91.1
rps12-clpP_p2 TTGCACCAACGGAAACCATA ATGTCAGCCACAGAAGCAAA 45.0 45.0 58.0 58.0 598-736 bp 1.0 91.1
rps12-clpP_p3 TGCACCAACGGAAACCATAA TATACGGCTCCTAAGGGGTG 45.0 55.0 58.0 58.0 1209 bp 0.166667 46.5
rps12-clpP_p4 TTGCACCAACGGAAACCATA TATACGGCTCCTAAGGGGTG 45.0 55.0 58.0 58.0 1210 bp 0.166667 46.5
rps12-clpP_p5 GATTGGCTGTCTTGCGTTTC TATACGGCTCCTAAGGGGTG 50.0 55.0 58.0 58.0 979 bp 0.166667 46.4