Hotspot

rpl33-rps18

LSC · 68213-68731 · 519 bp

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Pi 0.0132
MarkerSeek score 58.80
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
519
variable_sites
14
pi
0.0132
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACACCTAACCGA GGATTTACGAAAGAGCCGCT 47.6 50.0 57.8 58.3 635-672 bp 1.0 70.0
rpl33-rps18_p2 TCGACACAATACACCTAACCG GGATTTACGAAAGAGCCGCT 47.6 50.0 57.8 58.3 636-673 bp 1.0 70.0
rpl33-rps18_p3 CGACACAATACACCTAACCGA TGGATTTACGAAAGAGCCGC 47.6 50.0 57.8 58.6 636-673 bp 1.0 67.7
rpl33-rps18_p4 TCGACACAATACACCTAACCG TGGATTTACGAAAGAGCCGC 47.6 50.0 57.8 58.6 637-674 bp 1.0 67.7
rpl33-rps18_p5 CGACACAATACACCTAACCG GGATTTACGAAAGAGCCGCT 50.0 50.0 56.3 58.3 635-672 bp 1.0 66.1