Hotspot

trnT-UGU-trnL-UAA

LSC · 46415-47253 · 839 bp

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Pi 0.00302
MarkerSeek score 57.90
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
839
variable_sites
5
pi
0.00302
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 872-900 bp 1.0 80.0
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 904-932 bp 1.0 79.1
trnT-UGU-trnL-UAA_p3 AAGAAATAGGGAAAGCCGGC CCGTAGCGTCTACCGATTTC 50.0 55.0 58.2 58.2 928-956 bp 1.0 79.0
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 866-894 bp 1.0 78.8
trnT-UGU-trnL-UAA_p5 AAGAAATAGGGAAAGCCGGC CGTCTACCGATTTCGCCATA 50.0 50.0 58.2 57.6 922-950 bp 1.0 77.7