Hotspot

psaA-ycf3

LSC · 41223-42058 · 836 bp

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Pi 0.00662
MarkerSeek score 58.30
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
836
variable_sites
15
pi
0.00662
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 1004-1039 bp 1.0 81.5
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 986-1021 bp 1.0 81.5
psaA-ycf3_p3 AGAAATGACCCGGTTTAGCC ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 1021-1056 bp 1.0 81.4
psaA-ycf3_p4 AGAAATGACCCGGTTTAGCC GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 1003-1038 bp 1.0 81.4
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT CAGAGGCTTGGTTCGATCAA 50.0 50.0 57.9 57.9 1019-1054 bp 1.0 81.0