Hotspot

psbZ-trnG-GCC

LSC · 35165-35856 · 692 bp

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Pi 0.0116
MarkerSeek score 68.00
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
692
variable_sites
19
pi
0.0116
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGGTG CGTCTTCTCCTTGGCAAAGA 55.0 50.0 57.9 57.8 846-867 bp 1.0 83.5
psbZ-trnG-GCC_p2 CCCGTTGTTTTTGCTTCTCC CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.9 57.8 859-880 bp 1.0 83.3
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 849-870 bp 1.0 82.8
psbZ-trnG-GCC_p4 TTCTCCTGATGGTTGGGTGA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.3 57.8 845-866 bp 1.0 82.3
psbZ-trnG-GCC_p5 CTTCTCCTGATGGTTGGGTG TAAATACATTGCCTGGGCGG 55.0 50.0 57.9 58.3 885-906 bp 1.0 82.2