Hotspot

petN-psbM

LSC · 26660-27431 · 772 bp

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Pi 0.00941
MarkerSeek score 58.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
772
variable_sites
16
pi
0.00941
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GGCGGCATTAATGACAGTCT ATTGCTACTGCGCTGTTCAT 50.0 45.0 58.3 58.3 915-920 bp 1.0 77.2
petN-psbM_p2 GGCGGCATTAATGACAGTCT TATTGCTACTGCGCTGTTCA 50.0 45.0 58.3 57.6 916-921 bp 1.0 76.2
petN-psbM_p3 GGCGGCATTAATGACAGTCT TTGCTACTGCGCTGTTCATT 50.0 45.0 58.3 58.5 914-919 bp 1.0 75.4
petN-psbM_p4 GGCGGCATTAATGACAGTCT TGCTTTTATTGCTACTGCGC 50.0 45.0 58.3 57.5 922-927 bp 1.0 74.9
petN-psbM_p5 GGCGGCATTAATGACAGTCT GCTACTGCGCTGTTCATTCT 50.0 50.0 58.3 58.6 912-917 bp 1.0 74.2