Hotspot

rps16

LSC · 5370-6513 · 1144 bp

Back to Vasconcellea

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1144
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TACGAGTTATCCACACCCCC ACGGGTTCCAAAATAGAGGA 55.0 45.0 58.5 56.1 1204 bp 1.0 61.0
rps16_p2 TACGAGTTATCCACACCCCC TGTACCGATCCAACAATGACT 55.0 42.9 58.5 57.0 1256 bp 1.0 60.1
rps16_p3 TACGAGTTATCCACACCCCC ACGGGTTCCAAAATAGAGGAA 55.0 42.9 58.5 56.9 1204 bp 1.0 59.2
rps16_p4 CGAGTTATCCACACCCCCTT ACGGGTTCCAAAATAGAGGA 55.0 45.0 59.1 56.1 1202 bp 1.0 56.4
rps16_p5 TACGAGTTATCCACACCCCC ATGTACCGATCCAACAATGACT 55.0 40.9 58.5 57.5 1257 bp 1.0 56.0