Hotspot

trnS-GCU-trnS-CGA

LSC · 8531-9549 · 1019 bp

Back to Vasconcellea

Pi 0

MarkerSeek score 59.60

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 1019
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACCAAAAGACCCCT	50.0	40.9	58.0	58.1	1124-1133 bp	1.0	64.0
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACCAAAAGACCCCT	50.0	40.9	58.0	58.1	1123-1132 bp	1.0	64.0
trnS-GCU-trnS-CGA_p3	AGGAAACGGAAAGAGAGGGA	TGAATCAAACCAAAAGACCCCT	50.0	40.9	58.0	58.1	1183-1192 bp	1.0	63.8
trnS-GCU-trnS-CGA_p4	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCAAAAGACCCCT	50.0	40.9	58.2	58.1	1178-1187 bp	1.0	62.8
trnS-GCU-trnS-CGA_p5	CAATCCGACGCTTTAGTCCA	TGAATCAAACCAAAAGACCCC	50.0	42.9	58.0	56.6	1124-1133 bp	1.0	61.4