Hotspot

matK-trnK-UUU

LSC · 3698-4460 · 763 bp

Back to Vasconcellea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AAGTCGTGTTGCCGAAATCT	TCGGGTTGCTAACTCAATGG	45.0	50.0	58.1	57.9	855 bp	1.0	78.2
matK-trnK-UUU_p2	AAGTCGTGTTGCCGAAATCT	CGGGTTGCTAACTCAATGGT	45.0	50.0	58.1	58.2	854 bp	1.0	77.6
matK-trnK-UUU_p3	GAAGTCGTGTTGCCGAAATC	TCGGGTTGCTAACTCAATGG	50.0	50.0	57.8	57.9	856 bp	1.0	77.3
matK-trnK-UUU_p4	GAAGTCGTGTTGCCGAAATC	CGGGTTGCTAACTCAATGGT	50.0	50.0	57.8	58.2	855 bp	1.0	76.7
matK-trnK-UUU_p5	TATAGGAAGTCGTGTTGCCG	TCGGGTTGCTAACTCAATGG	50.0	50.0	57.4	57.9	861 bp	1.0	74.6