Hotspot

rpl16

LSC · 84239-86110 · 1872 bp

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Pi 0.0525

MarkerSeek score 80.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1872
variable_sites: 194
pi: 0.0525
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATCAATTTTCGAGCTCCGCT	ACGGAAAAGAGATTGCACGT	45.0	45.0	58.0	58.1	2296-2412 bp	1.0	90.3
rpl16_p2	CGAGCAATCGCACCAAAAAT	ACGGAAAAGAGATTGCACGT	45.0	45.0	58.0	58.1	2548-2664 bp	1.0	89.9
rpl16_p3	CGAGCAATCGCACCAAAAAT	AAAGAGATTGCACGTGTCGA	45.0	45.0	58.0	57.9	2543-2659 bp	1.0	89.7
rpl16_p4	CGAGCAATCGCACCAAAAAT	AAGAGATTGCACGTGTCGAA	45.0	45.0	58.0	57.9	2542-2658 bp	1.0	89.7
rpl16_p5	GTCGCGTTTTAGTTCTTGGC	ACGGAAAAGAGATTGCACGT	50.0	45.0	58.1	58.1	2530 bp	0.2	46.6