Hotspot

petD

LSC · 77521-79858 · 2338 bp

Back to Vanilla

Pi 0.0326

MarkerSeek score 57.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 2338
variable_sites: 93
pi: 0.0326
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GAAGTGTTAGTGTGGGCCAA	ACGAGTGGAATGAGCTTTGA	50.0	45.0	58.0	57.2	1987-2775 bp	0.6	65.2
petD_p2	ATTGCCTCTTCTTACTGCCG	ACGAGTGGAATGAGCTTTGA	50.0	45.0	58.0	57.2	1870-2717 bp	0.6	64.6
petD_p3	ATCATATCGGGGAGGAGCAA	TGCAGAGGAAGAGGATTTGG	50.0	50.0	58.0	57.2	2974 bp	0.2	41.4
petD_p4	GAAGTGTTAGTGTGGGCCAA	AGCTTTGAATCCTCGACACA	50.0	45.0	58.0	57.2	2763 bp	0.2	41.1
petD_p5	ATTGCCTCTTCTTACTGCCG	AGCTTTGAATCCTCGACACA	50.0	45.0	58.0	57.2	2705 bp	0.2	41.0