Hotspot

rps7-trnL-CAA

IRa · 138455-139496 · 1042 bp

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Pi 0.0857

MarkerSeek score 77.50

Primer available yes

Label rps7-trnL-CAA

Feature Metadata

label_name: rps7-trnL-CAA
feature_type: igs
region: IRa
length_bp: 1042
variable_sites: 201
pi: 0.0857
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-trnL-CAA_p1	ATGGCAGAGGCAAATAGAGC	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.0	58.0	1075-1113 bp	1.0	91.8
rps7-trnL-CAA_p2	ATGGCAGAGGCAAATAGAGC	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.0	58.0	1073-1111 bp	1.0	91.8
rps7-trnL-CAA_p3	GGCAGAGGCAAATAGAGCTT	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.0	58.0	1073-1111 bp	1.0	91.5
rps7-trnL-CAA_p4	GGCAGAGGCAAATAGAGCTT	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.0	58.0	1071-1109 bp	1.0	91.5
rps7-trnL-CAA_p5	ATGGCAGAGGCAAATAGAGC	GCGTGTCTACCATTTCACCA	50.0	50.0	58.0	58.2	1070-1108 bp	1.0	90.3