Hotspot

trnV-GAC-3'rps12

IRa · 136138-137104 · 967 bp

Back to Vanilla

Pi 0.0687

MarkerSeek score 78.40

Primer available yes

Label trnV-GAC-3'rps12

Feature Metadata

label_name: trnV-GAC-3'rps12
feature_type: igs
region: IRa
length_bp: 967
variable_sites: 102
pi: 0.0687
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-3'rps12_p1	CCTCAGCCTACGGGGTATTA	TCTACAACGCACTAGAACGC	55.0	50.0	58.0	58.0	1508-1875 bp	1.0	91.2
trnV-GAC-3'rps12_p2	CCTCAGCCTACGGGGTATTA	TCTCACACCGGGTAAATCCT	55.0	50.0	58.0	58.1	1432-1799 bp	1.0	91.2
trnV-GAC-3'rps12_p3	CCTCAGCCTACGGGGTATTA	CGAATACGGCTTTCCACAGA	55.0	50.0	58.0	58.0	1984-2351 bp	1.0	89.7
trnV-GAC-3'rps12_p4	CCTCAGCCTACGGGGTATTA	TTGTCGGCGATCCTTTACTG	55.0	50.0	58.0	58.0	1853 bp	0.2	47.9
trnV-GAC-3'rps12_p5	CGTCGCCTTGGTAAGCTATT	TTGTCGGCGATCCTTTACTG	50.0	50.0	58.1	58.0	1932 bp	0.2	47.5