Hotspot

ycf1-trnN-GUU

IRa · 127072-127607 · 536 bp

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Pi 0.0901

MarkerSeek score 79.50

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 536
variable_sites: 113
pi: 0.0901
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	GGTCGGCCCAATGCTAAATA	TCCTAATGGTCAAAGCGAGC	50.0	50.0	58.0	58.0	914 bp	0.2	47.6
ycf1-trnN-GUU_p2	AAAACCAGTTGTCGCTGCTA	TCCTAATGGTCAAAGCGAGC	45.0	50.0	58.0	58.0	846 bp	0.2	47.5
ycf1-trnN-GUU_p3	CCTAGTGAAAATGGGGGACG	TCCTAATGGTCAAAGCGAGC	55.0	50.0	58.0	58.0	1377 bp	0.2	47.5
ycf1-trnN-GUU_p4	CTAGTGAAAATGGGGGACGG	TCCTAATGGTCAAAGCGAGC	55.0	50.0	58.0	58.0	1376 bp	0.2	47.5
ycf1-trnN-GUU_p5	CCTTCTTCCATAACCCGAGC	TCCTAATGGTCAAAGCGAGC	55.0	50.0	58.0	58.0	812 bp	0.2	47.4