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psaC-rpl32

SSC · 118771-119358 · 588 bp

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Pi 0.0859

MarkerSeek score 83.90

Primer available yes

Label psaC-rpl32

Feature Metadata

label_name: psaC-rpl32
feature_type: igs
region: SSC
length_bp: 588
variable_sites: 113
pi: 0.0859
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rpl32_p1	ACATCCGTCCCAAGGTATCA	TGCTCTGCAATCACAATGGT	50.0	45.0	58.1	58.1	1247-1298 bp	0.8	82.3
psaC-rpl32_p2	ACATCCGTCCCAAGGTATCA	GTACCTGCTCTGCAATCACA	50.0	50.0	58.1	57.9	1252-1303 bp	0.8	82.2
psaC-rpl32_p3	GACCCATGCTACGAGTTGTT	TGCTCTGCAATCACAATGGT	50.0	45.0	57.9	58.1	1430-1461 bp	0.6	74.4
psaC-rpl32_p4	GACCCATGCTACGAGTTGTT	GTACCTGCTCTGCAATCACA	50.0	50.0	57.9	57.9	1435-1466 bp	0.6	74.3
psaC-rpl32_p5	ACAATCCTCGGTTCTTGGTG	TGCTCTGCAATCACAATGGT	50.0	45.0	57.8	58.1	1340 bp	0.2	45.8