Hotspot

ccsA-psaC

SSC · 118108-118524 · 417 bp

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Pi 0.106

MarkerSeek score 80.30

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 417
variable_sites: 88
pi: 0.106
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	ATTCGGTGGATTTCTTCGGG	AACAACTCGTAGCATGGGTC	50.0	50.0	58.0	57.9	1174-1329 bp	1.0	90.9
ccsA-psaC_p2	TGCTCTGCAATCACAATGGT	AACAACTCGTAGCATGGGTC	45.0	50.0	58.1	57.9	959-1114 bp	1.0	90.6
ccsA-psaC_p3	GTACCTGCTCTGCAATCACA	AACAACTCGTAGCATGGGTC	50.0	50.0	57.9	57.9	964-1119 bp	1.0	90.4
ccsA-psaC_p4	ATTCGGTGGATTTCTTCGGG	TGATACCTTGGGACGGATGT	50.0	50.0	58.0	58.1	1307-1331 bp	0.8	82.3
ccsA-psaC_p5	TGCTCTGCAATCACAATGGT	TGATACCTTGGGACGGATGT	45.0	50.0	58.1	58.1	1092-1116 bp	0.8	82.3