Hotspot

petB-petD

LSC · 77351-77520 · 170 bp

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Pi 0.0541

MarkerSeek score 78.00

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 21
pi: 0.0541
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CAGTTCATCGATGGTCAGCA	TGACACTTCCTTCGGGTTTG	50.0	50.0	58.0	58.0	783-812 bp	1.0	85.7
petB-petD_p2	GAAGTGTTAGTGTGGGCCAA	TGACACTTCCTTCGGGTTTG	50.0	50.0	58.0	58.0	530-551 bp	0.6	68.2
petB-petD_p3	CAGTTCATCGATGGTCAGCA	ACTGCGAAGACACATTGGTT	50.0	45.0	58.0	58.0	705 bp	0.2	47.6
petB-petD_p4	GAAGTGTTAGTGTGGGCCAA	ACTGCGAAGACACATTGGTT	50.0	45.0	58.0	58.0	444 bp	0.2	47.5
petB-petD_p5	ATTGCCTCTTCTTACTGCCG	ACTGCGAAGACACATTGGTT	50.0	45.0	58.0	58.0	386 bp	0.2	47.5