Hotspot

accD-psaI

LSC · 59461-60084 · 624 bp

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Pi 0.0901
MarkerSeek score 82.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
624
variable_sites
92
pi
0.0901
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.88
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GGTACCTTCCTCCTCCTTTG GAGGGAAAGTTCAAATCTATCA 55.0 36.4 56.9 53.5 624-673 bp 1.0 52.0
accD-psaI_p2 GGTACCTTCCTCCTCCTTTGA GAGGGAAAGTTCAAATCTATCA 52.4 36.4 58.5 53.5 624-673 bp 1.0 52.0
accD-psaI_p3 GGTACCTTCCTCCTCCTTTG GAGGGAAAGTTCAAATCTATCAT 55.0 34.8 56.9 54.1 624-673 bp 1.0 52.0
accD-psaI_p4 GGTACCTTCCTCCTCCTTTG AGGGAAAGTTCAAATCTATCA 55.0 33.3 56.9 52.1 623-672 bp 1.0 52.0
accD-psaI_p5 GGTACCTTCCTCCTCCTTTG AGGGAAAGTTCAAATCTATCAT 55.0 31.8 56.9 52.8 623-672 bp 1.0 52.0