Hotspot

rbcL-accD

LSC · 55326-56310 · 985 bp

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Pi 0.0394

MarkerSeek score 79.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 84
pi: 0.0394
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GACCCGGTAGATAAGCCAGA	ACTTACTAAACGTACACACAT	55.0	33.3	58.3	52.6	1051-1168 bp	1.0	50.1
rbcL-accD_p2	TCGATTTCGACCCGGTAGAT	ACTTACTAAACGTACACACAT	50.0	33.3	58.3	52.6	1059-1176 bp	1.0	50.1
rbcL-accD_p3	ACCCGGTAGATAAGCCAGAT	ACTTACTAAACGTACACACAT	50.0	33.3	57.6	52.6	1050-1167 bp	1.0	50.1
rbcL-accD_p4	AGCAAATGGAGCCCTGAATT	ACTTACTAAACGTACACACAT	45.0	33.3	57.8	52.6	1114-1231 bp	1.0	49.8
rbcL-accD_p5	CTAGCAAATGGAGCCCTGAA	ACTTACTAAACGTACACACAT	50.0	33.3	57.6	52.6	1116-1233 bp	1.0	49.8