Hotspot

atpB-rbcL

LSC · 52412-53846 · 1435 bp

Back to Vanilla

Pi 0.0734

MarkerSeek score 77.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1435
variable_sites: 156
pi: 0.0734
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCGACTGGAACACTTAGAG	GTCCACACAGTTGTCCATGT	55.0	50.0	58.0	58.0	1604-1983 bp	1.0	91.1
atpB-rbcL_p2	GCCGACTGGAACACTTAGAG	ACGAAACGATCTCTCCAACG	55.0	50.0	58.0	58.0	2048-2427 bp	1.0	90.1
atpB-rbcL_p3	AAAGCGGGTGCAGATCTATG	ACGAAACGATCTCTCCAACG	50.0	50.0	58.0	58.0	2155-2534 bp	1.0	89.8
atpB-rbcL_p4	TGCCCCGTGGAAAAATTACA	ACGAAACGATCTCTCCAACG	45.0	50.0	58.0	58.0	2233 bp	0.2	47.8
atpB-rbcL_p5	TGCCCCGTGGAAAAATTACA	GTCCACACAGTTGTCCATGT	45.0	50.0	58.0	58.0	1789 bp	0.2	47.7