Hotspot

rpoB-trnC-GCA

LSC · 28759-29790 · 1032 bp

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Pi 0.0591

MarkerSeek score 84.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1032
variable_sites: 132
pi: 0.0591
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTAATGCCGCGGGTACAT	TTACCACTTGGCTATGTCGC	45.0	50.0	58.0	58.0	1314-1887 bp	1.0	90.4
rpoB-trnC-GCA_p2	CAAATTAATGCCGCGGGTAC	TTACCACTTGGCTATGTCGC	50.0	50.0	57.9	58.0	1316-1889 bp	1.0	89.7
rpoB-trnC-GCA_p3	ATGCCGGGACTTTGCAATAT	TTACCACTTGGCTATGTCGC	45.0	50.0	57.9	58.0	1448-2021 bp	1.0	89.4
rpoB-trnC-GCA_p4	AATTAATGCCGCGGGTACAT	TGAATGATGCCCCTCCCTAT	45.0	50.0	58.0	57.9	1537-2453 bp	1.0	89.2
rpoB-trnC-GCA_p5	AATTAATGCCGCGGGTACAT	TGGGCCGGAATAAGAGAAGA	45.0	50.0	58.0	58.1	1961 bp	0.2	46.8