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atpH-atpI

LSC · 14749-16233 · 1485 bp

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Pi 0.0866

MarkerSeek score 66.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1485
variable_sites: 153
pi: 0.0866
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCCACGACCAGTCCATAAAT	CAGTTATCACGGCTGTTCGA	50.0	50.0	58.0	58.0	1626-2290 bp	0.8	83.1
atpH-atpI_p2	GCCACGACCAGTCCATAAAT	TCAGTTATCACGGCTGTTCG	50.0	50.0	58.0	58.0	1627-2291 bp	0.8	83.1
atpH-atpI_p3	ACGAGTGGATCTGCGAATTC	CAGTTATCACGGCTGTTCGA	50.0	50.0	58.1	58.0	1873-2510 bp	0.8	81.9
atpH-atpI_p4	GGTCTCGTGTCAATCGTGAA	CAGTTATCACGGCTGTTCGA	50.0	50.0	57.9	58.0	2028-2690 bp	0.8	81.5
atpH-atpI_p5	GGTCTCGTGTCAATCGTGAA	TCAGTTATCACGGCTGTTCG	50.0	50.0	57.9	58.0	2029-2691 bp	0.8	81.5