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matK-trnK-UUU

LSC · 3268-4447 · 1180 bp

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Pi 0.0854

MarkerSeek score 79.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1180
variable_sites: 156
pi: 0.0854
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACGATCATGAGCAAGTGAGT	TGGGTGGTTTGTTTTCCAGT	45.0	45.0	57.0	57.8	1353-1640 bp	1.0	81.7
matK-trnK-UUU_p2	TCGTGGAAAAACAATCGCAA	TGGGTGGTTTGTTTTCCAGT	40.0	45.0	56.9	57.8	1811-2098 bp	1.0	80.9
matK-trnK-UUU_p3	TTCGTGGAAAAACAATCGCA	TGGGTGGTTTGTTTTCCAGT	40.0	45.0	56.9	57.8	1812-2099 bp	1.0	80.9
matK-trnK-UUU_p4	AGAACATCTTTGACCCAGCA	TGGGTGGTTTGTTTTCCAGT	45.0	45.0	57.0	57.8	1778-2065 bp	0.8	74.2
matK-trnK-UUU_p5	AGAACATCTTTGACCCAGCA	TTGGGTTGCTAACTCAACGG	45.0	50.0	57.0	58.4	1603-1890 bp	0.8	73.1