Hotspot

rpl16

LSC · 83131-84768 · 1638 bp

Back to Vandopsis

Pi 0.0135

MarkerSeek score 66.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1638
variable_sites: 22
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGTCCAGAGATAAAGAAATCCGT	TCAAAGAAAAACGAACAATTAGA	39.1	26.1	57.3	53.2	1777-1822 bp	1.0	41.5
rpl16_p2	TGTCCAGAGATAAAGAAATCCGT	TCTTAATCAAAGAAAAACGAACA	39.1	26.1	57.3	53.2	1783-1828 bp	1.0	41.5
rpl16_p3	TGTCCAGAGATAAAGAAATCCGT	TCAAAGAAAAACGAACAATTAGAA	39.1	25.0	57.3	54.0	1777-1822 bp	1.0	41.5
rpl16_p4	TGTCCAGAGATAAAGAAATCCGT	TCTTAATCAAAGAAAAACGAACAA	39.1	25.0	57.3	54.0	1783-1828 bp	1.0	41.5
rpl16_p5	TGTCCAGAGATAAAGAAATCCGT	ATCAAAGAAAAACGAACAATTAGA	39.1	25.0	57.3	53.8	1778-1823 bp	1.0	41.5