Hotspot

ndhG

SSC · 116605-117119 · 515 bp

Back to Vandopsis

Pi 0.0121

MarkerSeek score 53.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 515
variable_sites: 6
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TACTGAATGTGACATTGGGT	GCCACAAGGTACATTGGACA	40.0	50.0	54.3	58.1	1232-1320 bp	1.0	54.0
ndhG_p2	TACTGAATGTGACATTGGGT	TGAGCCACAAGGTACATTGG	40.0	50.0	54.3	57.8	1235-1323 bp	1.0	53.2
ndhG_p3	TACTGAATGTGACATTGGGT	AGCCACAAGGTACATTGGAC	40.0	50.0	54.3	57.8	1233-1321 bp	1.0	53.2
ndhG_p4	TTACTGAATGTGACATTGGGT	GCCACAAGGTACATTGGACA	38.1	50.0	55.1	58.1	1233-1321 bp	1.0	52.9
ndhG_p5	CTTTACTGAATGTGACATTGGGT	GCCACAAGGTACATTGGACA	39.1	50.0	57.0	58.1	1235-1323 bp	1.0	52.3