Hotspot

accD-psaI

LSC · 59513-60372 · 860 bp

Back to Vandopsis

Pi 0.0116

MarkerSeek score 60.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 10
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGTTTCCTTCCCTTGAA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	991 bp	1.0	80.6
accD-psaI_p2	TTCGACCCAATTGTACCACG	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.2	58.0	1051 bp	1.0	79.1
accD-psaI_p3	CGACCCAATTGTACCACGTA	AAAGAAGCCATTGCGATTGC	50.0	45.0	57.6	58.0	1049 bp	1.0	77.8
accD-psaI_p4	TCAGCTACACGGTTTCCTTC	AAAGAAGCCATTGCGATTGC	50.0	45.0	57.5	58.0	998 bp	1.0	77.2
accD-psaI_p5	CACGGTTTCCTTCCCTTGAA	CGATTGCCGGAAAGACTAGG	50.0	55.0	58.0	58.4	978 bp	1.0	77.2