Hotspot

rps16-trnQ-UUG

LSC · 6154-7020 · 867 bp

Back to Vandopsis

Pi 0.0104
MarkerSeek score 64.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
867
variable_sites
9
pi
0.0104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 962-963 bp 1.0 79.7
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 939-940 bp 1.0 79.1
rps16-trnQ-UUG_p3 CCACAGCCAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 960-961 bp 1.0 79.0
rps16-trnQ-UUG_p4 ATCCACAGCCAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 966-967 bp 1.0 78.6
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 950-951 bp 1.0 78.6