primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TAAGAAGGGAAGCTCGTTGC	ATAAGACCGCGACTGATCCT	20	20	50.0	50.0	57.9	58.3	0.0	8.2	0.401136	4161	4280	270-272	1.000000	81.2
trnK-UUU_p2	trnK-UUU	2	CTTTTCCCACATCAGGCACT	ATAAGACCGCGACTGATCCT	20	20	50.0	50.0	58.1	58.3	0.0	8.2	0.391233	4161	4280	340-342	1.000000	80.6
trnK-UUU_p3	trnK-UUU	3	GACCCTTTTCCCACATCAGG	ATAAGACCGCGACTGATCCT	20	20	55.0	50.0	58.2	58.3	0.0	8.2	0.466950	4161	4280	344-346	1.000000	80.0
trnK-UUU_p4	trnK-UUU	4	TCGCAACCCAATCACTCTTT	ATAAGACCGCGACTGATCCT	20	20	45.0	50.0	57.7	58.3	0.0	8.2	0.576726	4161	4280	480-482	1.000000	78.2
trnK-UUU_p5	trnK-UUU	5	TTCGCAACCCAATCACTCTT	ATAAGACCGCGACTGATCCT	20	20	45.0	50.0	57.7	58.3	0.0	8.2	0.576726	4161	4280	481-483	1.000000	78.2
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCCTGAAGGAGTAGCGGATA	TCCGGGTTGCTAACTCAATG	20	20	50.0	50.0	57.0	57.9	0.0	0.0	1.152416	3286	4455	1317-1318	1.000000	73.3
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCCTGAAGGAGTAGCGGATA	CGGGTTGCTAACTCAATGGT	20	20	50.0	50.0	57.0	58.2	0.0	0.0	1.223250	3286	4455	1315-1316	1.000000	72.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	TCCTGAAGGAGTAGCGGATA	GATCCGGGTTGCTAACTCAA	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.441056	3286	4455	1319-1320	1.000000	71.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCCTGAAGGAGTAGCGGATA	TTGATCCGGGTTGCTAACTC	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.441056	3286	4455	1321-1322	1.000000	71.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCCTGAAGGAGTAGCGGATAT	TCCGGGTTGCTAACTCAATG	21	20	47.6	50.0	57.5	57.9	0.0	0.0	1.635457	3286	4455	1317-1318	1.000000	69.5
rps16_p1	rps16	1	CATTGAGTTAGCAACCCGGA	AAGTGAAATCCTTTCCGGGT	20	20	50.0	45.0	57.9	57.0	0.7	0.0	1.079851	4968	6153	1823-1850	1.000000	72.4
rps16_p2	rps16	2	ACCATTGAGTTAGCAACCCG	AAGTGAAATCCTTTCCGGGT	20	20	50.0	45.0	58.2	57.0	0.0	0.0	1.150684	4968	6153	1825-1852	1.000000	71.8
rps16_p3	rps16	3	CATTGAGTTAGCAACCCGGA	TGTATCGGTTCAGCCAATGA	20	20	50.0	45.0	57.9	56.9	0.7	0.0	1.177773	4968	6153	1765-1792	1.000000	71.6
rps16_p4	rps16	4	CATTGAGTTAGCAACCCGGA	GTATCGGTTCAGCCAATGACT	20	21	50.0	47.6	57.9	58.1	0.7	0.0	1.201596	4968	6153	1764-1791	1.000000	71.4
rps16_p5	rps16	5	ACCATTGAGTTAGCAACCCG	TGTATCGGTTCAGCCAATGA	20	20	50.0	45.0	58.2	56.9	0.0	0.0	1.248606	4968	6153	1767-1794	1.000000	71.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.139072	6154	7020	962-963	1.000000	79.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6154	7020	939-940	1.000000	79.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CCACAGCCAATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.232733	6154	7020	960-961	1.000000	79.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ATCCACAGCCAATCATGTCC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	57.9	57.8	0.0	16.9	0.274323	6154	7020	966-967	1.000000	78.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6154	7020	950-951	1.000000	78.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AATTCTTCCGTCAAGCCCTG	CCGGGGATAAAGGATTTGCA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.239514	27228	28889	1698-1817	1.000000	82.3
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	AATTCTTCCGTCAAGCCCTG	ACCGGGGATAAAGGATTTGC	20	20	50.0	50.0	58.2	57.9	0.0	6.3	0.240981	27228	28889	1699-1818	1.000000	82.3
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AATTCTTCCGTCAAGCCCTG	TCGAACCGGGGATAAAGGAT	20	20	50.0	50.0	58.2	58.2	0.0	6.3	0.376295	27228	28889	1703-1822	1.000000	81.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	AATTCTTCCGTCAAGCCCTG	TTCGAACCGGGGATAAAGGA	20	20	50.0	50.0	58.2	58.4	0.0	6.3	0.602812	27228	28889	1704-1823	1.000000	79.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AAATTCTTCCGTCAAGCCCT	CCGGGGATAAAGGATTTGCA	20	20	45.0	50.0	57.4	57.9	0.0	0.0	0.652547	27228	28889	1699-1818	1.000000	79.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	32539	33377	914-943	1.000000	82.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	32539	33377	893-922	1.000000	81.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	32539	33377	890-919	1.000000	81.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32539	33377	892-921	1.000000	81.4
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	32539	33377	911-940	1.000000	81.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTGGACCTTCCCTAAAATCCC	AGAAGGTCTACGGTTCGAGT	21	20	52.4	50.0	57.7	57.8	0.0	0.0	1.495969	51172	51874	837-846	1.000000	70.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGACCTTCCCTAAAATCCCAG	AGAAGGTCTACGGTTCGAGT	21	20	52.4	50.0	57.7	57.8	0.0	0.0	1.495969	51172	51874	835-844	1.000000	70.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGGACCTTCCCTAAAATCCCA	AGAAGGTCTACGGTTCGAGT	21	20	47.6	50.0	58.3	57.8	0.0	0.0	1.547270	51172	51874	836-845	1.000000	70.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGACCTTCCCTAAAATCCCA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	56.5	57.8	0.0	0.0	1.713010	51172	51874	835-844	1.000000	68.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGACCTTCCCTAAAATCCC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	56.5	57.8	0.0	0.0	1.713010	51172	51874	836-845	1.000000	68.9
accD-psaI_p1	accD-psaI	1	CACGGTTTCCTTCCCTTGAA	AAAGAAGCCATTGCGATTGC	20	20	50.0	45.0	58.0	58.0	0.0	1.6	0.024770	59513	60372	991-991	1.000000	80.6
accD-psaI_p2	accD-psaI	2	TTCGACCCAATTGTACCACG	AAAGAAGCCATTGCGATTGC	20	20	50.0	45.0	58.2	58.0	4.4	1.6	0.202146	59513	60372	1051-1051	1.000000	79.1
accD-psaI_p3	accD-psaI	3	CGACCCAATTGTACCACGTA	AAAGAAGCCATTGCGATTGC	20	20	50.0	45.0	57.6	58.0	4.4	1.6	0.367459	59513	60372	1049-1049	1.000000	77.8
accD-psaI_p4	accD-psaI	4	TCAGCTACACGGTTTCCTTC	AAAGAAGCCATTGCGATTGC	20	20	50.0	45.0	57.5	58.0	0.0	1.6	0.451039	59513	60372	998-998	1.000000	77.2
accD-psaI_p5	accD-psaI	5	CACGGTTTCCTTCCCTTGAA	CGATTGCCGGAAAGACTAGG	20	20	50.0	55.0	58.0	58.4	0.0	5.0	0.452422	59513	60372	978-978	1.000000	77.2
petA-psbJ_p1	petA-psbJ	1	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.721854	63996	64945	1062-1062	1.000000	75.0
petA-psbJ_p2	petA-psbJ	2	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.729457	63996	64945	1063-1063	1.000000	74.9
petA-psbJ_p3	petA-psbJ	3	GAGTCCAAGGCCTTCTGTTC	TATTCCGGGTTGGGTTCATC	20	20	55.0	50.0	58.2	57.3	17.3	0.0	0.906632	63996	64945	1087-1087	1.000000	73.5
petA-psbJ_p4	petA-psbJ	4	GAGTCCAAGGCCTTCTGTTC	CTATTCCGGGTTGGGTTCAT	20	20	55.0	50.0	58.2	57.3	17.3	0.0	0.914235	63996	64945	1088-1088	1.000000	73.4
petA-psbJ_p5	petA-psbJ	5	GTCCAAGGCCTTCTGTTCTT	TATTCCGGGTTGGGTTCATC	20	20	50.0	50.0	57.7	57.3	17.3	0.0	0.999758	63996	64945	1085-1085	1.000000	72.7
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GCTGGGGTTATAGTTGAAGCA	20	21	50.0	47.6	57.9	58.0	6.4	0.0	1.052003	65703	66803	1264-1298	1.000000	72.3
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GGGTTATAGTTGAAGCAGCCA	20	21	50.0	47.6	57.9	58.0	6.4	0.0	1.052003	65703	66803	1260-1294	1.000000	72.3
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTGAAGCAGC	20	21	50.0	47.6	57.9	58.0	6.4	0.0	1.052003	65703	66803	1262-1296	1.000000	72.3
psbE-petL_p4	psbE-petL	4	ACTGACGAATAACCAACCCG	GGGTTATAGTTGAAGCAGCCA	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.089618	65703	66803	1273-1307	1.000000	72.0
psbE-petL_p5	psbE-petL	5	ACTGACGAATAACCAACCCG	TGGGGTTATAGTTGAAGCAGC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.089618	65703	66803	1275-1309	1.000000	72.0
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028080	73270	73975	846-880	1.000000	84.6
clpP-psbB_p2	clpP-psbB	2	CTTTTGGAACACCAATGGGC	CAACCAGAAACCAGAGCTGT	20	20	50.0	50.0	57.8	58.0	3.6	0.0	0.195454	73270	73975	792-826	1.000000	83.3
clpP-psbB_p3	clpP-psbB	3	ACCCAAACTGCATCTTCCTC	CAACCAGAAACCAGAGCTGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.233704	73270	73975	832-866	1.000000	83.0
clpP-psbB_p4	clpP-psbB	4	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	73270	73975	768-802	1.000000	83.0
clpP-psbB_p5	clpP-psbB	5	TCCTCTCCAGGATTCCGAAA	CAACCAGAAACCAGAGCTGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.274827	73270	73975	817-851	1.000000	82.7
psbB-psbT_p1	psbB-psbT	1	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	20	21	45.0	47.6	57.7	57.6	1.9	0.0	1.666612	75503	75882	554-574	1.000000	70.2
psbB-psbT_p2	psbB-psbT	2	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGA	20	21	45.0	47.6	57.7	57.3	1.9	0.0	1.943119	75503	75882	553-573	1.000000	68.0
psbB-psbT_p3	psbB-psbT	3	GCCAGAACCTTGTTCAGAGA	TGGAACTTTAGGTGGTTCTCG	20	21	50.0	47.6	57.4	57.6	9.4	0.0	1.947091	75503	75882	567-587	1.000000	67.9
psbB-psbT_p4	psbB-psbT	4	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	20	21	50.0	47.6	57.4	57.6	0.0	0.0	2.040194	75503	75882	516-536	1.000000	67.5
psbB-psbT_p5	psbB-psbT	5	GCCAGAACCTTGTTCAGAGA	GGAACTTTAGGTGGTTCTCGA	20	21	50.0	47.6	57.4	57.3	9.4	0.0	2.223597	75503	75882	566-586	1.000000	65.7
rpoA-rps11_p1	rpoA-rps11	1	ACGCTTGCTGTCTACTCTTG	AAGAGATGCGGCATTACGAG	20	20	50.0	50.0	57.9	58.1	0.0	17.8	0.213597	80788	80871	277-285	1.000000	79.1
rpoA-rps11_p2	rpoA-rps11	2	ACGCTTGCTGTCTACTCTTG	GAAGAGATGCGGCATTACGA	20	20	50.0	50.0	57.9	58.1	0.0	17.8	0.218747	80788	80871	278-286	1.000000	79.1
rpoA-rps11_p3	rpoA-rps11	3	GGACAGAACGAAACGACCAT	AAGAGATGCGGCATTACGAG	20	20	50.0	50.0	58.2	58.1	0.0	17.8	0.349903	80788	80871	304-312	1.000000	78.0
rpoA-rps11_p4	rpoA-rps11	4	GGACAGAACGAAACGACCAT	GAAGAGATGCGGCATTACGA	20	20	50.0	50.0	58.2	58.1	0.0	17.8	0.355052	80788	80871	305-313	1.000000	77.9
rpoA-rps11_p5	rpoA-rps11	5	ACGCTTGCTGTCTACTCTTG	CGTACGTGATGTAACACCCA	20	20	50.0	50.0	57.9	57.6	0.0	7.2	0.422587	80788	80871	226-234	1.000000	77.7
rpl16_p1	rpl16	1	TGTCCAGAGATAAAGAAATCCGT	TCAAAGAAAAACGAACAATTAGA	23	23	39.1	26.1	57.3	53.2	0.0	0.0	11.509400	83131	84768	1777-1822	1.000000	41.5
rpl16_p2	rpl16	2	TGTCCAGAGATAAAGAAATCCGT	TCTTAATCAAAGAAAAACGAACA	23	23	39.1	26.1	57.3	53.2	0.0	0.0	11.509400	83131	84768	1783-1828	1.000000	41.5
rpl16_p3	rpl16	3	TGTCCAGAGATAAAGAAATCCGT	TCAAAGAAAAACGAACAATTAGAA	23	24	39.1	25.0	57.3	54.0	0.0	0.0	11.719503	83131	84768	1777-1822	1.000000	41.5
rpl16_p4	rpl16	4	TGTCCAGAGATAAAGAAATCCGT	TCTTAATCAAAGAAAAACGAACAA	23	24	39.1	25.0	57.3	54.0	0.0	0.0	11.719503	83131	84768	1783-1828	1.000000	41.5
rpl16_p5	rpl16	5	TGTCCAGAGATAAAGAAATCCGT	ATCAAAGAAAAACGAACAATTAGA	23	24	39.1	25.0	57.3	53.8	0.0	0.0	11.920465	83131	84768	1778-1823	1.000000	41.5
ycf2_p1	ycf2	1	TCGAATCCTACTGAGAGGTCC	CCTTTGTTCCGGTCATGGAA	21	20	52.4	50.0	58.1	58.1	0.0	0.0	1.162140	90396	90515	284-284	1.000000	74.1
ycf2_p2	ycf2	2	TCTGGGTTCGAATCCTACTGA	CCTTTGTTCCGGTCATGGAA	21	20	47.6	50.0	57.9	58.1	27.2	0.0	1.191625	90396	90515	291-291	1.000000	73.8
ycf2_p3	ycf2	3	TGGGTTCGAATCCTACTGAGA	CCTTTGTTCCGGTCATGGAA	21	20	47.6	50.0	57.9	58.1	27.2	0.0	1.191625	90396	90515	289-289	1.000000	73.8
ycf2_p4	ycf2	4	TGGTCAGAACTTCATCTGGG	CCTTTGTTCCGGTCATGGAA	20	20	50.0	50.0	56.8	58.1	4.6	0.0	1.254380	90396	90515	305-305	1.000000	73.1
ycf2_p5	ycf2	5	GGTCAGAACTTCATCTGGGT	CCTTTGTTCCGGTCATGGAA	20	20	50.0	50.0	56.8	58.1	4.6	0.0	1.256564	90396	90515	304-304	1.000000	73.1
ycf1-rpl32_p1	ycf1-rpl32	1	GCTTCCTTCGTAACTCCAGG	TTCGTTCTCAGTCGGAGTCT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.117996	111751	112213	1288-1342	1.000000	84.5
ycf1-rpl32_p2	ycf1-rpl32	2	GCTTCCTTCGTAACTCCAGG	CTTTTTGACTGTCCGGTGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.049747	111751	112213	944-944	0.500000	59.6
ycf1-rpl32_p3	ycf1-rpl32	3	AAAGCGAGCCCTTTCATTCA	CTTTTTGACTGTCCGGTGGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.128507	111751	112213	1129-1129	0.500000	59.0
ycf1-rpl32_p4	ycf1-rpl32	4	AAGCGAGCCCTTTCATTCAA	CTTTTTGACTGTCCGGTGGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.128507	111751	112213	1128-1128	0.500000	59.0
ycf1-rpl32_p5	ycf1-rpl32	5	TCAAAGCGAGCCCTTTCATT	CTTTTTGACTGTCCGGTGGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.128507	111751	112213	1131-1131	0.500000	59.0
ndhG_p1	ndhG	1	TACTGAATGTGACATTGGGT	GCCACAAGGTACATTGGACA	20	20	40.0	50.0	54.3	58.1	16.5	22.9	3.845551	116605	117119	1232-1320	1.000000	54.0
ndhG_p2	ndhG	2	TACTGAATGTGACATTGGGT	TGAGCCACAAGGTACATTGG	20	20	40.0	50.0	54.3	57.8	16.5	22.9	3.948664	116605	117119	1235-1323	1.000000	53.2
ndhG_p3	ndhG	3	TACTGAATGTGACATTGGGT	AGCCACAAGGTACATTGGAC	20	20	40.0	50.0	54.3	57.8	16.5	22.9	3.950210	116605	117119	1233-1321	1.000000	53.2
ndhG_p4	ndhG	4	TTACTGAATGTGACATTGGGT	GCCACAAGGTACATTGGACA	21	20	38.1	50.0	55.1	58.1	16.5	22.9	3.980394	116605	117119	1233-1321	1.000000	52.9
ndhG_p5	ndhG	5	CTTTACTGAATGTGACATTGGGT	GCCACAAGGTACATTGGACA	23	20	39.1	50.0	57.0	58.1	16.5	22.9	4.060671	116605	117119	1235-1323	1.000000	52.3