Hotspot

ycf4

Genome · 64823-65386 · 564 bp

Back to Vandenboschia

Pi 0.00177

MarkerSeek score 53.20

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: Genome
length_bp: 564
variable_sites: 1
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TCTTTAGGCTTTTGGGGAGT	TCTCCTTCTATCCTATCTCCT	45.0	42.9	56.3	53.4	646 bp	1.0	40.1
ycf4_p2	TCTTTAGGCTTTTGGGGAGT	TTCTCCTTCTATCCTATCTCCT	45.0	40.9	56.3	54.3	647 bp	1.0	40.1
ycf4_p3	ATCTTTAGGCTTTTGGGGAGT	TCTCCTTCTATCCTATCTCCT	42.9	42.9	56.9	53.4	647 bp	1.0	40.1
ycf4_p4	TCTTTAGGCTTTTGGGGAGT	ATTCTCCTTCTATCCTATCTCCT	45.0	39.1	56.3	54.9	648 bp	1.0	40.1
ycf4_p5	ATCTTTAGGCTTTTGGGGAGT	TTCTCCTTCTATCCTATCTCCT	42.9	40.9	56.9	54.3	648 bp	1.0	40.1