Hotspot

rps16

Genome · 10587-11654 · 1068 bp

Back to Vandenboschia

Pi 0.000936

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1068
variable_sites: 1
pi: 0.000936
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAACCGAGCCCATTGGATTC	TGGTCTCTTTCACCAGAATCA	50.0	42.9	58.5	56.6	1161 bp	1.0	56.5
rps16_p2	AAACCGAGCCCATTGGATTC	TTGGTCTCTTTCACCAGAATCA	50.0	40.9	58.5	57.3	1162 bp	1.0	54.2
rps16_p3	TAAACCGAGCCCATTGGATT	TGGTCTCTTTCACCAGAATCA	45.0	42.9	57.2	56.6	1162 bp	1.0	54.1
rps16_p4	CCGAGCCCATTGGATTCTC	TGGTCTCTTTCACCAGAATCA	57.9	42.9	58.0	56.6	1158 bp	1.0	52.6
rps16_p5	AAACCGAGCCCATTGGATTC	TGGTCTCTTTCACCAGAATCAT	50.0	40.9	58.5	57.1	1161 bp	1.0	52.5