Hotspot

rpoC1

Genome · 27489-30225 · 2737 bp

Back to Vandenboschia

Pi 0.000731
MarkerSeek score 56.60
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
Genome
length_bp
2737
variable_sites
2
pi
0.000731
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 AGAAAAGACTTCCTTGATCCT TTTGGCGTAGACTTGAGACG 38.1 50.0 54.1 57.9 2873 bp 1.0 40.8
rpoC1_p2 ACTAGAAAAGACTTCCTTGATCCT TTTGGCGTAGACTTGAGACG 37.5 50.0 57.1 57.9 2876 bp 1.0 40.1
rpoC1_p3 AGAAAAGACTTCCTTGATCCTT TTTGGCGTAGACTTGAGACG 36.4 50.0 55.0 57.9 2873 bp 1.0 40.1
rpoC1_p4 AGAAAAGACTTCCTTGATCCT GCGTAGACTTGAGACGAGAG 38.1 55.0 54.1 57.6 2869 bp 1.0 40.1
rpoC1_p5 ACTAGAAAAGACTTCCTTGATCC TTTGGCGTAGACTTGAGACG 39.1 50.0 55.7 57.9 2876 bp 1.0 40.1