Hotspot

psbD

Genome · 41053-42114 · 1062 bp

Back to Vandenboschia

Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	GGTTTCCTGATCTCGACCAC	AGGACACCTAAAAACAGTTGAGT	55.0	39.1	58.0	57.9	1152 bp	1.0	55.1
psbD_p2	GTGGTTTCCTGATCTCGACC	AGGACACCTAAAAACAGTTGAGT	55.0	39.1	58.0	57.9	1154 bp	1.0	55.1
psbD_p3	GTTTCCTGATCTCGACCACC	AGGACACCTAAAAACAGTTGAGT	55.0	39.1	58.0	57.9	1151 bp	1.0	55.1
psbD_p4	CCTGTGGTTTCCTGATCTCG	AGGACACCTAAAAACAGTTGAGT	55.0	39.1	58.0	57.9	1157 bp	1.0	55.0
psbD_p5	CGACCACCCAGAGATAGAGT	AGGACACCTAAAAACAGTTGAGT	55.0	39.1	57.9	57.9	1139 bp	1.0	54.7