Hotspot

chlB

Genome · 11801-13342 · 1542 bp

Back to Vandenboschia

Pi 0.0013

MarkerSeek score 59.40

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 1542
variable_sites: 2
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	TGATTCTGGTGAAAGAGACCA	TAGATTTCCCTTTGACGCGG	42.9	50.0	56.6	58.0	1684 bp	1.0	60.5
chlB_p2	TGATTCTGGTGAAAGAGACCAA	TAGATTTCCCTTTGACGCGG	40.9	50.0	57.3	58.0	1684 bp	1.0	58.3
chlB_p3	ATGATTCTGGTGAAAGAGACCA	TAGATTTCCCTTTGACGCGG	40.9	50.0	57.1	58.0	1685 bp	1.0	56.6
chlB_p4	TGATTCTGGTGAAAGAGACCA	CCTTTGACGCGGAAGAAAAA	42.9	45.0	56.6	57.2	1676 bp	1.0	54.6
chlB_p5	TGATTCTGGTGAAAGAGACCA	ATTTCCCTTTGACGCGGAAG	42.9	50.0	56.6	58.8	1681 bp	1.0	54.0