Hotspot

rrn16-trnV-GAC

Genome · 137730-138407 · 678 bp

Back to Vandenboschia

Pi 0.00147
MarkerSeek score 59.60
Primer available yes
Label rrn16-trnV-GAC

Feature Metadata

label_name
rrn16-trnV-GAC
feature_type
igs
region
Genome
length_bp
678
variable_sites
1
pi
0.00147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnV-GAC_p1 GAGCCAGGATCAAACTCTCC TCTCCTCATGGGTAAGCGAT 55.0 50.0 57.7 57.9 824 bp 1.0 76.8
rrn16-trnV-GAC_p2 AGCCAGGATCAAACTCTCCA TCTCCTCATGGGTAAGCGAT 50.0 50.0 58.3 57.9 823 bp 1.0 76.7
rrn16-trnV-GAC_p3 GAGCCAGGATCAAACTCTCC GGTGCAAGTCATCAGTTCGA 55.0 50.0 57.7 58.2 739 bp 1.0 75.9
rrn16-trnV-GAC_p4 GAGCCAGGATCAAACTCTCC TCCTCATGGGTAAGCGATCA 55.0 50.0 57.7 58.2 822 bp 1.0 75.8
rrn16-trnV-GAC_p5 AGCCAGGATCAAACTCTCCA GGTGCAAGTCATCAGTTCGA 50.0 50.0 58.3 58.2 738 bp 1.0 75.8