Hotspot

trnV-GAC-rrn16

Genome · 98537-99214 · 678 bp

Back to Vandenboschia

Pi 0.00147

MarkerSeek score 59.60

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: Genome
length_bp: 678
variable_sites: 1
pi: 0.00147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	TCTCCTCATGGGTAAGCGAT	GAGCCAGGATCAAACTCTCC	50.0	55.0	57.9	57.7	824 bp	1.0	76.8
trnV-GAC-rrn16_p2	TCTCCTCATGGGTAAGCGAT	AGCCAGGATCAAACTCTCCA	50.0	50.0	57.9	58.3	823 bp	1.0	76.7
trnV-GAC-rrn16_p3	GGTGCAAGTCATCAGTTCGA	GAGCCAGGATCAAACTCTCC	50.0	55.0	58.2	57.7	739 bp	1.0	75.9
trnV-GAC-rrn16_p4	TCCTCATGGGTAAGCGATCA	GAGCCAGGATCAAACTCTCC	50.0	55.0	58.2	57.7	822 bp	1.0	75.8
trnV-GAC-rrn16_p5	GGTGCAAGTCATCAGTTCGA	AGCCAGGATCAAACTCTCCA	50.0	50.0	58.2	58.3	738 bp	1.0	75.8