Hotspot

trnL-CAA-trnF-GAA

Genome · 54352-54770 · 419 bp

Back to Vandenboschia

Pi 0.00239
MarkerSeek score 56.40
Primer available yes
Label trnL-CAA-trnF-GAA

Feature Metadata

label_name
trnL-CAA-trnF-GAA
feature_type
igs
region
Genome
length_bp
419
variable_sites
1
pi
0.00239
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-trnF-GAA_p1 AATCCGCTGGTCTTTGTAGC GACTTGAACTGGTGACACGA 50.0 50.0 58.3 57.8 530 bp 1.0 76.3
trnL-CAA-trnF-GAA_p2 AATCCGCTGGTCTTTGTAGC ACTTGAACTGGTGACACGAG 50.0 50.0 58.3 57.8 529 bp 1.0 76.3
trnL-CAA-trnF-GAA_p3 AATCCGCTGGTCTTTGTAGC AGACTTGAACTGGTGACACG 50.0 50.0 58.3 57.8 531 bp 1.0 76.3
trnL-CAA-trnF-GAA_p4 AATCCGCTGGTCTTTGTAGC TCTACCGACTGAGCTATCCC 50.0 55.0 58.3 57.7 492 bp 1.0 75.9
trnL-CAA-trnF-GAA_p5 AATCCGCTGGTCTTTGTAGC ATCCTCTGCTCTACCGACTG 50.0 55.0 58.3 58.3 501 bp 1.0 75.6