Hotspot

rps4-trnL-CAA

Genome · 53010-53783 · 774 bp

Back to Vandenboschia

Pi 0.00129

MarkerSeek score 49.00

Primer available yes

Label rps4-trnL-CAA

Feature Metadata

label_name: rps4-trnL-CAA
feature_type: igs
region: Genome
length_bp: 774
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-CAA_p1	GGGTCCTCTGTAACGAGACA	GCGTCTACCATTTCGCCATA	55.0	50.0	58.5	58.1	820 bp	1.0	75.3
rps4-trnL-CAA_p2	TAACGGGGTCCTCTGTAACG	GCGTCTACCATTTCGCCATA	55.0	50.0	58.8	58.1	825 bp	1.0	72.4
rps4-trnL-CAA_p3	GGGGTCCTCTGTAACGAGAC	GCGTCTACCATTTCGCCATA	60.0	50.0	59.2	58.1	821 bp	1.0	69.5
rps4-trnL-CAA_p4	GGGTCCTCTGTAACGAGACA	TACCATTTCGCCATATCCCC	55.0	50.0	58.5	57.1	815 bp	1.0	69.5
rps4-trnL-CAA_p5	GGGTCCTCTGTAACGAGACA	AATTTGATTGAGTCCGCAGC	55.0	45.0	58.5	57.1	838 bp	1.0	69.0