Hotspot

petN-psbM

Genome · 34355-35844 · 1490 bp

Back to Vandenboschia

Pi 0.000671

MarkerSeek score 59.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 1490
variable_sites: 1
pi: 0.000671
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTTTGGGGAAGGA	TCGCATTCATAGCTACTGCA	50.0	45.0	58.0	57.1	1610-1614 bp	1.0	72.8
petN-psbM_p2	TCGTAGTTTGGGGAAGGAGT	TCGCATTCATAGCTACTGCA	50.0	45.0	58.0	57.1	1608-1612 bp	1.0	72.8
petN-psbM_p3	ACTCGTAGTTTGGGGAAGGA	CGCATTCATAGCTACTGCAC	50.0	50.0	58.0	57.1	1609-1613 bp	1.0	72.4
petN-psbM_p4	TCGTAGTTTGGGGAAGGAGT	CGCATTCATAGCTACTGCAC	50.0	50.0	58.0	57.1	1607-1611 bp	1.0	72.4
petN-psbM_p5	CGTAGTTTGGGGAAGGAGTG	TCGCATTCATAGCTACTGCA	55.0	45.0	57.9	57.1	1607-1611 bp	1.0	72.2