Hotspot

atpH-atpI

Genome · 20699-21323 · 625 bp

Back to Vandenboschia

Pi 0.0016

MarkerSeek score 59.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 625
variable_sites: 1
pi: 0.0016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	TTTGCAACTTTAGCTGCAGC	50.0	45.0	58.1	57.9	717 bp	1.0	78.2
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TTTGCAACTTTAGCTGCAGC	45.0	45.0	57.9	57.9	727 bp	1.0	77.8
atpH-atpI_p3	AGCAGAAATCAAGGGGTTCG	TTTGCAACTTTAGCTGCAGC	50.0	45.0	58.2	57.9	700 bp	1.0	77.6
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TTTGCAACTTTAGCTGCAGC	50.0	45.0	57.8	57.9	726 bp	1.0	77.3
atpH-atpI_p5	CAATGCCTTCTACTGCCTGA	TTTGCAACTTTAGCTGCAGC	50.0	45.0	57.6	57.9	797 bp	1.0	75.7