Hotspot

trnH-GTG-psbA

Genome · 5817-6277 · 461 bp

Back to Vandenboschia

Pi 0.00434

MarkerSeek score 58.30

Primer available yes

Label trnH-GTG-psbA

Feature Metadata

label_name: trnH-GTG-psbA
feature_type: igs
region: Genome
length_bp: 461
variable_sites: 2
pi: 0.00434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	GATTGAACCCGCGCATAATG	TGGGCTGATATCATCAACCG	50.0	50.0	58.0	57.5	636 bp	1.0	75.6
trnH-GTG-psbA_p2	GATTGAACCCGCGCATAATG	GGGCTGATATCATCAACCGT	50.0	50.0	58.0	57.4	635 bp	1.0	75.6
trnH-GTG-psbA_p3	GATTGAACCCGCGCATAATG	GCTGATATCATCAACCGTGC	50.0	50.0	58.0	57.0	633 bp	1.0	72.3
trnH-GTG-psbA_p4	GATTGAACCCGCGCATAATG	TGAAGCTCCTTCTGTAAACGG	50.0	47.6	58.0	58.0	547 bp	1.0	71.7
trnH-GTG-psbA_p5	TTGCCTTAATCCACTTGGCT	TGGGCTGATATCATCAACCG	45.0	50.0	57.4	57.5	603 bp	1.0	71.0