Hotspot

ycf1

SSC · 122447-128020 · 5574 bp

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Pi 0.041

MarkerSeek score 65.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5574
variable_sites: 227
pi: 0.041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CATTCCTGACAGCCCTTTCA	TCGTCGTGGTTGGACTCTAT	50.0	50.0	57.8	58.2	1113 bp	0.5	57.0
ycf1_p2	TCCATCGCTTCGAATCGAAA	TCGTCGTGGTTGGACTCTAT	45.0	50.0	57.7	58.2	1059 bp	0.5	56.3
ycf1_p3	TTCCATCGCTTCGAATCGAA	TCGTCGTGGTTGGACTCTAT	45.0	50.0	57.7	58.2	1060 bp	0.5	56.3
ycf1_p4	CGGTGGATACCTCTTGTTCC	TCGTCGTGGTTGGACTCTAT	55.0	50.0	57.7	58.2	780 bp	0.5	56.2
ycf1_p5	AATGGGCGATGGTATTCTGC	TCGTCGTGGTTGGACTCTAT	50.0	50.0	58.4	58.2	659 bp	0.5	55.5