Hotspot

ndhF

SSC · 109409-111667 · 2259 bp

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Pi 0.0345

MarkerSeek score 62.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 78
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AATACCCCTTTCGGATCGGA	GCCCAGTACCCCTTTCTTTT	50.0	50.0	58.2	57.7	2921 bp	0.5	55.9
ndhF_p2	ATACCCCTTTCGGATCGGAA	GCCCAGTACCCCTTTCTTTT	50.0	50.0	58.2	57.7	2920 bp	0.5	55.9
ndhF_p3	TACCCCTTTCGGATCGGAAT	GCCCAGTACCCCTTTCTTTT	50.0	50.0	58.2	57.7	2919 bp	0.5	55.9
ndhF_p4	CCCTTTCGGATCGGAATTCA	GCCCAGTACCCCTTTCTTTT	50.0	50.0	57.7	57.7	2916 bp	0.5	55.0
ndhF_p5	TACCCCTTTCGGATCGGAAT	TTTTAACGCTGCCCAGTACC	50.0	50.0	58.2	58.5	2929 bp	0.5	54.6