Hotspot

ndhD

SSC · 114214-115716 · 1503 bp

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Pi 0.02

MarkerSeek score 55.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 30
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGAGTTGGGACCCAAAAG	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2221-2233 bp	1.0	82.3
ndhD_p2	CTAACGAAGCATGGGGATCC	GATACCCTGGGACGGATGTA	55.0	55.0	58.1	58.0	2243-2255 bp	1.0	82.0
ndhD_p3	TTGGAGTTGGGACCCAAAAG	TGTGAATCTGCCTGTCCAAC	50.0	50.0	57.9	58.1	2145-2157 bp	1.0	81.5
ndhD_p4	TTGGAGTTGGGACCCAAAAG	AAATGATACCCTGGGACGGA	50.0	50.0	57.9	58.1	2225-2237 bp	1.0	81.4
ndhD_p5	TTCAGCAATTGTGGCGTCTA	GATACCCTGGGACGGATGTA	45.0	55.0	57.8	58.0	2134 bp	0.5	58.5