Hotspot

ccsA-ndhD

SSC · 113989-114213 · 225 bp

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Pi 0.0561
MarkerSeek score 74.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
225
variable_sites
12
pi
0.0561
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GGGGTCAATCTATTAGGAATAGGAC GGTTGAAGTTATTCTATCTAATTCT 44.0 28.0 58.1 52.7 314-325 bp 1.0 45.8
ccsA-ndhD_p2 TGGGGTCAATCTATTAGGAATAGG GGTTGAAGTTATTCTATCTAATTCT 41.7 28.0 57.0 52.7 315-326 bp 1.0 45.7
ccsA-ndhD_p3 TGGATATGTTATTTTGGGGTCA GGTTGAAGTTATTCTATCTAATTCT 36.4 28.0 55.1 52.7 329-340 bp 1.0 45.5
ccsA-ndhD_p4 TGGATATGTTATTTTGGGGTCAA GGTTGAAGTTATTCTATCTAATTCT 34.8 28.0 55.9 52.7 329-340 bp 1.0 45.5
ccsA-ndhD_p5 TTGGATATGTTATTTTGGGGTCA GGTTGAAGTTATTCTATCTAATTCT 34.8 28.0 55.9 52.7 330-341 bp 1.0 45.5