Hotspot

rpl32-trnL-UAG

SSC · 112353-112835 · 483 bp

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Pi 0.0738
MarkerSeek score 79.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
483
variable_sites
35
pi
0.0738
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGTTTCTTTGTAGGACAAACA AGCGTGTCTACCGATTTCAC 33.3 50.0 53.2 58.0 557-911 bp 1.0 44.8
rpl32-trnL-UAG_p2 AGTTTCTTTGTAGGACAAACA CAGCGTGTCTACCGATTTCA 33.3 50.0 53.2 58.0 558-912 bp 1.0 44.8
rpl32-trnL-UAG_p3 AAGTTTCTTTGTAGGACAAACA AGCGTGTCTACCGATTTCAC 31.8 50.0 54.1 58.0 558-912 bp 1.0 44.8
rpl32-trnL-UAG_p4 AGTTTCTTTGTAGGACAAACAA AGCGTGTCTACCGATTTCAC 31.8 50.0 54.1 58.0 557-911 bp 1.0 44.8
rpl32-trnL-UAG_p5 AGTTTCTTTGTAGGACAAACA TCCTAAGAGCAGCGTGTCTA 33.3 50.0 53.2 57.9 567-921 bp 1.0 44.7