Hotspot

rpl36-infA

LSC · 80372-80469 · 98 bp

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Pi 0.0543

MarkerSeek score 76.90

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 98
variable_sites: 5
pi: 0.0543
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CTATAATACGCCCCCGCCTA	ACGCATACTGCCAGGAGATA	55.0	50.0	58.5	58.3	268-274 bp	1.0	76.7
rpl36-infA_p2	CTATAATACGCCCCCGCCTA	ATACGCATACTGCCAGGAGA	55.0	50.0	58.5	58.3	270-276 bp	1.0	76.7
rpl36-infA_p3	CTATAATACGCCCCCGCCTA	TACGCATACTGCCAGGAGAT	55.0	50.0	58.5	58.3	269-275 bp	1.0	76.7
rpl36-infA_p4	TATAATACGCCCCCGCCTAC	ACGCATACTGCCAGGAGATA	55.0	50.0	58.8	58.3	267-273 bp	1.0	74.4
rpl36-infA_p5	TATAATACGCCCCCGCCTAC	TACGCATACTGCCAGGAGAT	55.0	50.0	58.8	58.3	268-274 bp	1.0	74.4